Mouse Anti-Fc P329G LALA Recombinant Antibody (clone P329G LALA)
CAT#: PABJ-0115
This product is a Mouse antibody that recognizes Human Fc P329G LALA.
Tested Data
SDS-PAGE
Figure 1 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in SDS-PAGE
SDS-PAGE analysis of PABJ-0115 in non-reduced (Lane 2) and reduced (Lane 1) conditions. Gel stained for 30 minutes with Coomassie Blue. As a result of different β-mercaptoethanol-reduced proteins (Heavy chain and Light chain) migrate as about 50 kDa and 25 kDa, respectively.
HPLC
Figure 2 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in HPLC
The purity of this antibody is> 95% as determined by SEC-HPLC.
WB
Figure 3 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in WB
Western blot analysis was performed by loading recombinant human IgG1 with Fc P329G LALA onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least 1 hour. Membranes were probed with Anti-Human Fc P329G LALA Antibody (PABJ-0115) at a concentration of 1 μg/mL overnight at 4°C on a rocking platform. Then probed with Goat anti-Mouse IgG HRP secondary antibody at a dilution of 1:8,000 for one hour. Chemiluminescent was detected.
Lane 1: Reducing antigen (0.1 μg)
Lane 2: Reducing antigen (0.3 μg)
Lane 3: Reducing antigen (0.6 μg)
ELISA
Figure 4 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in ELISA
ELISA analysis of PABJ-0115 was performed by coating with recombinant human IgG1 with Fc P329G LALA. Then blocked with BSA and incubated with Anti-Human Fc P329G LALA Antibody (PABJ-0115) at a starting concentration of 2.5 ng/mL. The HRP-conjugated goat anti-Mouse IgG as a secondary antibody. Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
DB
Figure 5 Mouse Anti-Fc P329G LALA Antibody (PABJ-0115) in DB
Dot Blot analysis of PABJ-0115 was performed by coating with recombinant human IgG1 with Fc P329G LALA.
PABJ-0115 incubation concentration: 2 ng/μL.
The secondary antibody: HRP-Goat Anti-Mouse IgG (H+L)
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human Fc P329G LALA
- Species Reactivity
- Human
- Clone
- P329G LALA
- Applications
- ELISA, FuncS
Product Property
- Purification
- Protein A/G Purified
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Buffer
- PBS
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been reported for use in Enzyme-linked Immunosorbent Assay, Functional Assay.
Target
- Alternative Names
- Fc P329G; LALA; P329G Fc mutation
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To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# PABJ-0115, RRID: AB_3111605)
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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